Intravital microscopy (IVM) is an advanced imaging technique that allows real-time, high-resolution visualization of biological processes within living organisms at the cellular and subcellular levels. Through the use of fluorescent labeling strategies and specialized imaging modalities such as multiphoton or spinning disk confocal microscopy, IVM enables the study of dynamic interactions within native tissue environments, including cell migration, vascular dynamics, and immune responses, under physiological or pathological conditions.
This approach provides essential information on spatiotemporal mechanisms that cannot be captured in fixed or in vitro systems, bridging the gap between molecular biology and whole-organism physiology.
Why move toward intravital microscopy ?
By enabling longitudinal imaging in the same animal, IVM significantly increases data reliability and statistical power, while reducing the number of animals required and the overall costs of the experiment. This continuity of observation allows for a more accurate and integrated understanding of dynamic biological mechanisms. While ex vivo analyses remain essential for hypothesis formulation and preliminary characterization, in vivo imaging represents a decisive step in the validation of biological phenomena.
IVM Advantages
- reliability
- animal use reduction
- Biological process validation
- cost reduction
Why our window ?
Intravital imaging can place significant physical stress on the animal, which prompted us to develop our lightweight and flexible PDMS window to mitigate these effects and improve experimental performance.
Compared to conventional titanium or glass models, this window is up to ten times lighter, offering greater comfort and reducing pain thanks to its flexible and soft structure. Its disposable format eliminates the risks associated with reuse, improving both safety and reproducibility. In addition, the refractive index of our PDMS, which is very close to that of water, allows for optimal imaging by long working distance two-photon microscope objectives, while the integrated injection port allows for convenient in situ manipulations during experiments.
Key Features
- High transparency
- Biocompatible
- Super light-weight
- wide field
- Single use
- 5' surgery
Super fast surgery
The failure of a surgical procedure is often due to the prolonged duration of the operation. In order to benefit the researcher, the animal, and the quality of the results, we have sought to reduce the duration of the procedure as much as possible. Our approach eliminates the need for sutures, ensures high tolerance, and allows access to the subcutaneous area in less than five minutes, from the first incision to the sealing of the window.
Visualize what microscopy could not reach before
Request Sample
We provide ready-to-use samples upon request to help you configure your imaging conditions. Simply fill out the form below to get contacted!